Coding

Part:BBa_K5035009:Design

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-10-02)


AI_4 Generated Reductive Dehalogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1359
    Illegal BamHI site found at 1371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AI_4 was given a hexahistidine tag to allow for nickel column protein purification.


Source

References

https://www.biorxiv.org/content/10.1101/2024.07.01.600583v1